University of Washington: Academic Personnel: School of Medicine: Medicine: CardiologyLocation Seattle, WA, United States
Open DateMay 07, 2024
DescriptionA postdoctoral position is available in the Division of Cardiology, Department of Medicine at the University of Washington to study the role of mitochondria-containing extracellular vesicles (MitoEV) in sterile inflammation associated with heart failure (HF) and type 2 diabetes (T2D).
This is a full-time Postdoctoral Scholar position on an annual, 12-month appointment. Postdoctoral scholars are represented by UAW 4121 and are subject to the collective bargaining agreement, unless agreed exclusion criteria apply. For more information, please visit the University of Washington Labor Relations website.
The project pursues investigations in the immune-modulating roles of circulating MitoEV in sterile inflammation in chronic conditions such as HF and T2D. Specifically, studies are carried out to study the regulation and mechanism of MitoEV release from peripheral immune cells in the setting of chronic inflammation, and the tissue-specific targets of proinflammatory MitoEV. Approaches include live-cell confocal and electron microscopy, flow analysis, mRNA/protein analyses, and genetic modifications by CRISPR-knock using cell-based and animal models. Experience in confocal microscopy and mouse models is highly desirable.
ResponsibilitiesDesign and perform cell-based experiments (50%):Generation of gene-deletions monocyte line(s) by CRISPRVolume-metric Flow analysis to quantify released MitoEVConfocal and electron microscopy of subcellular structures (e.g. mitochondria-containing endosomes)Functional and anatomical assessment of mitochondria and lysosome using biochemical assaysFunctional assessment of mitophagy/autophagy by live-cell imaging and flow analysisDevelop mouse models of MitoEV-induced inflammation (50%):Generate gene-knock out mouse line(s) and maintain mouse colonies.Venous injection of isolated MitoEVSmall animal nuclear imaging studies to determine tissue tropism of injected MitoEVTissue analysis of inflammationTrans-thoracic echocardiogram to determine LV functionsThe University of Washington is a rich environment for research in cardiovascular biology and disease. The Pacific Northwest is a pleasant place to live. Competitive salary. Opportunities for post-doc level grants, such as T32, F32, and AHA post-doc fellowship.
The base salary range for this position will be $5,705 per month, commensurate with experience and qualifications, or as mandated by a U.S. Department of Labor prevailing wage determination.
QualificationsThe successful candidate must have a Ph.D. or equivalent degree relevant to biological science and a strong publication record, particularly first-authorships. Prefer applicants with predilection toward an academic career.
Application InstructionsPlease submit a cover letter and CV as well as the names/emails for three references through Interfolio. With questions, you may contact Dennis Wang, MD, PhD, Division of Cardiology, University of Washington, ******.
Application ProcessThis institution is using Interfolio's Faculty Search to conduct this search. Applicants to this position receive a free Dossier account and can send all application materials, including confidential letters of recommendation, free of charge.
University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, creed, religion, national origin, sex, sexual orientation, marital status, pregnancy, genetic information, gender identity or expression, age, disability, or protected veteran status.
To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or ******.
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