Johnson & Johnson is recruiting a Proteomics Scientist, Cell Engineering located in Spring House, PA.
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The Cell Engineering & Analytical Sciences group within biologics development is seeking a highly skilled and motivated Proteomics Scientist to join our research and development team. You will play a crucial role in understanding cellular pathways and networks for optimizing recombinant protein production in engineered cell lines, using data-driven systems biology approaches. We are a pioneering mass spectrometry laboratory, equipped with over a dozen instruments, robotic workbenches for sample automation and powerful bioinformatic software and cloud computing resources for data interpretation. We work in an exciting, interdisciplinary environment and collaborate with multi-functional teams, including cell line development and cell culture process development, to provide expert guidance on proteomics-related aspects of cellular expression. Your expertise in protein analysis techniques will help drive the advancement of our biopharmaceutical pipeline. Key Responsibilities: Develop and implement proteomics strategies to support CHO cell engineering of complex biotherapeutic modalities. With the aim of optimizing protein expression levels, protein quality, and glycosylation patterns. Apply sophisticated liquid chromatography-mass spectrometry (LC-MS/MS) for protein identification, quantification, and post-translational modification analysis. Use biostatistics and bioinformatics algorithms to integrate multiple levels of biological information together with proteomics data, for new hypothesis generation, Troubleshoot technical issues and propose innovative solutions to enhance analytical capabilities. Stay up to date with the latest advancements in proteomics and cell engineering, develop new methods and publish in peer reviewed journals. Prepare and present scientific findings and recommendations to internal stakeholders, project teams, and senior management. Education: A Ph.D. in life sciences or a related field with 0-3 years of experience, MS with 3 years, or a Bachelors degree with 6 years is required Experience and Skills: Required: Strong background and hands-on experience in proteomics techniques, including sample preparation techniques, mass spectrometry-based analysis, and data analysis is required Proficiency in mass spectrometers method development (especially expertise in timsTOF & Orbitrap Eclipse instrumentation) and associated software for data acquisition and analysis (e.g. MaxQuant, MSFragger, DIA-NN, R programming, Cytoscape) is required Solid understanding of cell biology and recombinant protein expression is required Familiarity with cell culture techniques and genetic engineering tools for cell line development is required Demonstrated ability to work independently and collaboratively in a multidisciplinary team environment is required Excellent problem-solving skills and attention to detail is required Strong written and oral communication skills, with the ability to effectively present scientific findings and recommendations is required Preferred: Experience in high-throughput proteomics workflows and automation is preferred Knowledge of statistical analysis tools for data interpretation including multi-omics data sets. Applying systems biology approaches to generating hypothesis from -omics data is preferred Prior experience in the biopharmaceutical industry or related fields is preferred Experience in characterizing protein post-translational modifications i.e., ubiquitination, phosphorylation, and acetylation, and protein-protein interaction is preferred Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.
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