Scientist I (Recombinant Dna & Flow Cytometry)

Details of the offer

Skills Needed 1 year industry experience.Antibody production is major skills DNA recombinant DNA technology is must have.Should have FACS, analyzing the data using FlowJ DNA/RNA handling and other molecular biology techniques like PCR and qPCR must have these. ELISA and MSD for quantification for protein is preferred Tissue culture, transfection skillsGood to have cell line development preferred but not needed.Transfection, primer design, generation of target cell lines.Downstream protein purification later at midsize level.
Job Description We are currently seeking a highly motivated and passionate scientist in cellular immunology to join our team in Immunology Discovery department based in Cambridge MA.The candidate will focus on making therapeutic molecules using recombinant DNA technology, expression/purification of recombinant proteins, testing purified proteins in functional cellular assays, including basic cell biology and biochemical skills.The position offers a unique opportunity for a talented scientist to work in a dynamic, innovative, and multi-disciplinary environment to develop their career at the interface of basic research and early drug discovery.The scientist will be responsible for conceptualization, designing strategies to make therapeutic reagents, execution and analysis with the overall aim of promoting innovative drug discovery.The position requires ability to work independently, have excellent technical, written and oral communication skills and work very well in a collaborative team environment. Qualifications and Requirements The applicant must have a bachelor's degree in Biological Science or related field with 2-3 years of experience in biotech or pharma set up.Extensive hands-on experience in recombinant DNA technology, design, expression and purification of recombinant monoclonal antibodies.
Motivated to execute well-thought and well-planned in vitro experiments using the tool reagents generated.Hands-on experience in multi-color flow cytometry with both, handling flow cytometer (Canto, MACS Quant) and independently analyzing the data using analytical software such as FlowJo is required.
Proficient in carrying out ELISA/MSD to quantitate pro-inflammatory proteins.Basic cell biology and biochemical skills: Tissue culture, Transfection, Cell line generation, SDS-PAGE, Immunoprecipitation, Western blotting etc.Knowledge of DNA/RNA handling and other molecular biology techniques like PCR and qPCR.Hands on experience in carrying out size exclusion chromatography (SEC) and mass spectrometry. About US Tech Solutions: US Tech Solutions is a global staff augmentation firm providing a wide range of talent on-demand and total workforce solutions.
To know more about US Tech Solutions, please visit www.ustechsolutions.com.
US Tech Solutions is an Equal Opportunity Employer.
All qualified applicants will receive consideration for employment without regard to race, colour, religion, sex, sexual orientation, gender identity,
national origin, disability, or status as a protected veteran.


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